Quantifying Y chromosome loss in primary and metastatic prostate cancer by chromosome painting.

Autor: Sai Harisha Rajanala, Romina Ghale, Subhiksha Nandakumar, Kalyani Chadalavada, Gwo-Shu Mary Lee, Konrad H Stopsack, Yu Chen, Gouri J Nanjangud, Goutam Chakraborty, Philip W Kantoff
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: PLoS ONE, Vol 19, Iss 4, p e0301989 (2024)
Druh dokumentu: article
ISSN: 1932-6203
24665754
DOI: 10.1371/journal.pone.0301989
Popis: Somatic Y chromosome loss in hematopoietic cells is associated with higher mortality in men. However, the status of the Y chromosome in cancer tissue is not fully known due to technical limitations, such as difficulties in labelling and sequencing DNA from the Y chromosome. We have developed a system to quantify Y chromosome gain or loss in patient-derived prostate cancer organoids. Using our system, we observed Y chromosome loss in 4 of the 13 (31%) patient-derived metastatic castration-resistant prostate cancer (mCRPC) organoids; interestingly, loss of Yq (long arm of the Y chromosome) was seen in 38% of patient-derived organoids. Additionally, potential associations were observed between mCRPC and Y chromosome nullisomy. The prevalence of Y chromosome loss was similar in primary and metastatic tissue, suggesting that Y chromosome loss is an early event in prostate cancer evolution and may not a result of drug resistance or organoid derivation. This study reports quantification of Y chromosome loss and gain in primary and metastatic prostate cancer tissue and lays the groundwork for further studies investigating the clinical relevance of Y chromosome loss or gain in mCRPC.
Databáze: Directory of Open Access Journals
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