Isolation and Identification of Antioxidant Peptides Derived from Cricket (Gryllus bimaculatus) Protein Fractions

Autor:	Olumide Oluwatoyosi Fashakin, Pipat Tangjaidee, Kridsada Unban, Wannaporn Klangpetch, Tabkrich Khumsap, Korawan Sringarm, Saroat Rawdkuen, Suphat Phongthai
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	cricket protein fraction (CPF) antioxidant peptides fractionation protein hydrolysate bioactive peptide Science
Zdroj:	Insects, Vol 14, Iss 8, p 674 (2023)
Druh dokumentu:	article
ISSN:	2075-4450
DOI:	10.3390/insects14080674
Popis:	Crickets contain high protein content that can be used to improve nutrition but are less exploited. This study was conducted to isolate different Cricket Protein Fractions including albumin, globulin, glutelin, and prolamin. All fractions were characterized and hydrolyzed by commercial enzymes. The results showed that the glutelin fractions had the highest extraction yields with 53.9 ± 2.12% (p < 0.05). Moreover, glutelin hydrolysate fraction prepared by Alcalase with a 16.35 ±0.29% hydrolysis degree was selected for further purification because of their high antioxidant activities, including ABTS radical-scavenging activity (0.44–0.55 µmol Trolox eq./g) and metal chelating activity (1721.99–1751.71 µmol EDTA eq./g). Two active fractions, GA-1 (
Databáze:	Directory of Open Access Journals
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