Passengers screening for SARS-CoV-2 infection at Rome Fiumicino Airport: a strategy aimed at limiting the spreading of the infection through COVID-free air flights revealed the airport role as a formidable sentinel center for monitoring pandemic trends and viral variants circulation

Autor: Alessandra Amendola, Claudia Minosse, Fabrizio Carletti, Elena Specchiarello, Eleonora Lalle, Licia Bordi, Concetta Castilletti, Federica Forbici, Maria Pisciotta, Fabrizio Rossi, Tiziano Scarparo, Anna Rosa Garbuglia, Francesco Vaia
Jazyk: English<br />Italian
Rok vydání: 2022
Předmět:
Zdroj: Microbiologia Medica, Vol 37, Iss 1 (2022)
Druh dokumentu: article
ISSN: 2280-6423
DOI: 10.4081/mm.2022.10682
Popis: Background and aims: At the Fiumicino Rome Airport, passengers can undergo the antigen or molecular test for SARS-CoV-2 at the COVID-19 Test Area (CTA), in compliance with the boarding regulations on air flights and those for entering the destination countries. The aim of this study was to describe the adaptability and usefulness of using rapid molecular tests designed for point of care (POC) in a context such as the CTA of Rome Fiumicino airport, by describing the volume and activities performed in the last period of the state of emergency in Italy, July 10, 2021- March 31, 2022. Materials and methods: Rapid molecular tests for SARS-CoV-2 were carried out with ID NOWTM COVID-19 assay on the ID NOWTM platform using dry swabs collected from passengers (or airport staff) at the CTA. Swabs were stored at room temperature and analyzed within 1-2 hours of collection. Sequencing for SARS-CoV-2 variants of concern (VOC) identification was carried out on swabs dipped in universal transport medium (UTM), after new swab gathering from consenting passengers resulted positive with the rapid molecular assay. A proficiency panel prepared with the viral isolate 2019-nCoV/Italy INMI1 was used to evaluate analytical sensitivity; clinical sensitivity was tested by performing real-time polymerase chain reaction (RT-PCR) tests on 50 swabs obtained from passengers who previously resulted positive to ID NOWTM COVID-19 assay. Results: In total, 14632 rapid molecular tests for SARS-CoV-2 were performed at CTA and 5.6% resulted positive. Sequence analysis of samples with Ct value
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