Hyperinsulinemia Can Cause Kidney Disease in the IGT Stage of OLETF Rats via the INS/IRS-1/PI3-K/Akt Signaling Pathway

Autor: Yi Zhang, Shaohua Yang, Xiao Cui, Juhong Yang, Miaoyan Zheng, Junya Jia, Fei Han, Xiaoyun Yang, Jingyu Wang, Zhenhong Guo, Bai Chang, Baocheng Chang
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Journal of Diabetes Research, Vol 2019 (2019)
Druh dokumentu: article
ISSN: 2314-6745
2314-6753
DOI: 10.1155/2019/4709715
Popis: Aims. We investigated the changes of renal structure and its function in normal glucose tolerance (NGT), impaired glucose tolerance (IGT), diabetes mellitus (DM), and diabetic kidney disease (DKD) stages in OLETF rats and explored the role of the INS/IRS-1/PI3-K/Akt signaling pathway. Methods. OLETF rats were assigned into four groups on the basis of OGTT results and 24 h urinary microalbumin: NGT, IGT, DM, and DKD groups. The changes of renal structure and function and the corresponding pathological changes were observed. The absorption of albumin and the expression of megalin, cubilin, IRS-1, PI3-K, and Akt in NRK-52E cells were measured after being stimulated by different concentrations of insulin. Results. In the IGT group, the index which reflects the function of renal tubule-like N-acetyl-β-glucosaminidase, neutrophil gelatinase-associated lipocalin, retinol-binding protein, and cystatin C was higher than those in the control group and the NGT group (P
Databáze: Directory of Open Access Journals
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