Efficient Generation of Transgenic Buffalos (Bubalus bubalis) by Nuclear Transfer of Fetal Fibroblasts Expressing Enhanced Green Fluorescent Protein

Autor:	Fenghua Lu, Chan Luo, Nan Li, Qingyou Liu, Yingming Wei, Haiying Deng, Xiaoli Wang, Xiangping Li, Jianrong Jiang, Yanfei Deng, Deshun Shi
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	Medicine Science
Zdroj:	Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
Druh dokumentu:	article
ISSN:	2045-2322
DOI:	10.1038/s41598-018-25120-5
Popis:	Abstract The possibility of producing transgenic cloned buffalos by nuclear transfer of fetal fibroblasts expressing enhanced green fluorescent protein (EGFP) was explored in this study. When buffalo fetal fibroblasts (BFFs) isolated from a male buffalo fetus were transfected with pEGFP-N1 (EGFP is driven by CMV and Neo is driven by SV-40) by means of electroporation, Lipofectamine-LTX and X-tremeGENE, the transfection efficiency of electroporation (35.5%) was higher than Lipofectamine-LTX (11.7%) and X-tremeGENE (25.4%, P
Databáze:	Directory of Open Access Journals
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