Comparison of the protocols for obtaining platelet-rich plasma in dogs: a cellular study
| Autor: | André William Masseaux Vidal Júnior, Ricardo Siqueira da Silva, Ana Paula Lopes Marques, Heloísa Justen Moreira de Souza |
|---|---|
| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Ciência Rural, Vol 50, Iss 3 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1678-4596 0103-8478 |
| DOI: | 10.1590/0103-8478cr20180843 |
| Popis: | ABSTRACT: This study aimed to evaluate two protocols (PA and PB) that are used to obtain canine platelet-rich plasma (PRP) for cellularity. Twenty healthy dogs were used. Blood samples were collected and placed in two tubes of 3.2% sodium citrate. PA used double centrifugation at 210 x g, and 370 x g and PB used double centrifugation with 140 x g and 330 x g. The PRP samples from the protocols were examined in terms of their platelet, erythrocyte, and leukocyte count in the Neubauer chamber, differential leukocyte count and platelet morphological observation in blood smears. Data (mean and standard deviation) were analyzed with the 95% probability t-test (P 0.05). PA and PB presented low erythrocyte concentration (p> 0.05), and PA had more leukocytes (p |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|