Ladder-shape melting temperature isothermal amplification of nucleic acids

Autor:	Deguo Wang, Yongzhen Wang, Meng Zhang, Yongqing Zhang, Juntao Sun, Chunmei Song, Fugang Xiao, Yuan Ping, Chen Pan, Yushan Hu, Chaoqun Wang, Yanhong Liu
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	isothermal amplification ladder-shape melting temperature isothermal amplification (LMTIA) loop-mediated isothermal amplification (LAMP) melting curve nucleic acids detection primer design Biology (General) QH301-705.5
Zdroj:	BioTechniques, Vol 71, Iss 1, Pp 358-369 (2021)
Druh dokumentu:	article
ISSN:	1940-9818 0736-6205
DOI:	10.2144/btn-2020-0173
Popis:	A novel method, termed ladder-shape melting temperature isothermal amplification (LMTIA), was developed in this study. As a proof of concept, one pair of primers or two pairs of nested primers and a thermostable DNA polymerase were employed to amplify the internal transcribed spacer of Oryza sativa with the ladder-shape melting temperature curve. Our results demonstrated that the LMTIA assay with nested primers was 50-fold more sensitive than the LAMP assay with the same level of specificity. The LMTIA method has the potential to be used for the prevention and control of emerging epidemics caused by different types of pathogens.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/9e9617ec7dad403cb22902011b123b9f Zobrazit plný text záznamu View record in DOAJ