| Autor: |
Neal M. Rao, Joseph Capri, Whitaker Cohn, Maram Abdaljaleel, Lucas Restrepo, Jeffrey A. Gornbein, William H. Yong, David S. Liebeskind, Julian P. Whitelegge |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Frontiers in Neurology, Vol 8 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1664-2295 |
| DOI: |
10.3389/fneur.2017.00427 |
| Popis: |
IntroductionThe specific protein composition of stroke-causing emboli is unknown. Because ischemic stroke has a varied etiology, it is possible that the composition of the thrombus from which an embolus originated will have distinctive molecular characteristics reflective of the underlying pathophysiology. We used mass spectrometry to evaluate the protein composition of retrieved emboli from patients with differing stroke etiologies and correlated the protein levels to serum predictors of atherosclerosis.MethodsEmboli from 20 consecutive acute stroke patients were retrieved by thrombectomy during routine stroke care. Thrombus proteins were extracted, digested, and multidimensional fractionation of peptides was performed. Fractionated peptides underwent nano-liquid chromatography with tandem mass spectrometry. Spectra were searched using Mascot software in which results with p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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