Three-Dimensional Scaffold from Decellularized Human Gingiva for Cell Cultures: Glycoconjugates and Cell Behavior
| Autor: | Seyed Ali Banihashem Rad, Javad Baharara, Mojtaba Cheravi, Khadijeh Nejad Shahrokh Abady, Nasser Mahdavi Shahri, Jina Khayat Zadeh, Somayeh Naderi, Ahmad Reza Bahrami |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Cell Journal, Vol 15, Iss 2, Pp 166-175 (2013) |
| Druh dokumentu: | article |
| ISSN: | 2228-5806 2228-5814 |
| Popis: | Objective: We studied both the presence of some carbohydrate compounds in a three-dimensional (3D) matrix harvested from human gingiva and the cell behavior in this matrix.Materials and Methods: In this experimental research, in order to prepare 3D scaffolds, human palatal gingival biopsies were harvested and physically decellularized by freeze-thawing and sodium dodecyl sulfate (SDS). The scaffolds were placed within the rings of blastema tissues obtained from a pinna rabbit, in vitro. We evaluated the presence of glycoconjugatesand cellular behavior according to histological, histochemical and spectrophotometry techniques at one, two and three weeks after culture. One-way analysis of variance (ANOVA)comparedthe groups.Results: Extracellular matrix (ECM) remained after decellularization of tissue with 1% SDS. Glycoconjugate contents decreased meaningfully at a higher SDS concentration (p |
| Databáze: | Directory of Open Access Journals |
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