| Autor: |
Olaya Amor-Gutiérrez, Giulia Selvolini, M. Teresa Fernández-Abedul, Alfredo de la Escosura-Muñiz, Giovanna Marrazza |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Sensors, Vol 20, Iss 8, p 2349 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1424-8220 |
| DOI: |
10.3390/s20082349 |
| Popis: |
Nowadays, food allergy is a very important health issue, causing adverse reactions of the immune system when exposed to different allergens present in food. Because of this, the development of point-of-use devices using miniaturized, user-friendly, and low-cost instrumentation has become of outstanding importance. According to this, electrochemical aptasensors have been demonstrated as useful tools to quantify a broad variety of targets. In this work, we develop a simple methodology for the determination of β-lactoglobulin (β-LG) in food samples using a folding-based electrochemical aptasensor built on poly-L-lysine modified graphite screen-printed electrodes (GSPEs) and an anti-β-lactoglobulin aptamer tagged with methylene blue (MB). This aptamer changes its conformation when the sample contains β-LG, and due to this, the spacing between MB and the electrode surface (and therefore the electron transfer efficiency) also changes. The response of this biosensor was linear for concentrations of β-LG within the range 0.1–10 ng·mL−1, with a limit of detection of 0.09 ng·mL−1. The biosensor was satisfactorily employed for the determination of spiked β-LG in real food samples. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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