Hydrogen sulfide inhibits skeletal muscle ageing by up‐regulating autophagy through promoting deubiquitination of adenosine 5’‐monophosphate (AMP)‐activated protein kinase α1 via ubiquitin specific peptidase 5
Autor: | Jia‐He Yang, Jun Gao, Ya‐Qi E, Li‐Jie Jiao, Ren Wu, Qiu‐Yi Yan, Zi‐Yi Wei, Guo‐Liang Yan, Jin‐Long Liang, Hong‐Zhu Li |
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Jazyk: | angličtina |
Rok vydání: | 2024 |
Předmět: | |
Zdroj: | Journal of Cachexia, Sarcopenia and Muscle, Vol 15, Iss 5, Pp 2118-2133 (2024) |
Druh dokumentu: | article |
ISSN: | 2190-6009 2190-5991 |
DOI: | 10.1002/jcsm.13560 |
Popis: | Abstract Background Hydrogen sulfide (H2S), the third gasotransmitter discovered, regulates a variety of physiological functions. Whether H2S alleviates skeletal muscle ageing by regulating autophagy has not been reported. Methods Mice were administered 150 mg/kg/day of D‐galactose (D‐gal), and C2C12 myotubes were cultured in 20 g/L D‐gal to induce ageing. Sodium hydrosulfide (NaHS) was employed as an exogenous donor in the treatment group. The intracellular concentration of H2S was quantified by the 7‐azido‐4‐methylcoumarin fluorescence probe. The proteins involved in the ubiquitin‐mediated degradation of AMPKα1 were detected by liquid chromatography tandem mass spectrometry (LC–MS/MS) and co‐immunoprecipitation (Co‐IP). S‐sulfhydration of USP5 was tested by a biotin‐switch assay. Associated proteins were analysed by western blot. Results NaHS was found to effectively restore the H2S content in both ageing gastrocnemius (+91.89%, P |
Databáze: | Directory of Open Access Journals |
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