The Effect of Ozone Treatment on Metabolite Profile of Germinating Barley

Autor: Xue Dong, Litao Sun, Manjree Agarwal, Garth Maker, Yitao Han, Xiangyang Yu, Yonglin Ren
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Foods, Vol 11, Iss 9, p 1211 (2022)
Druh dokumentu: article
ISSN: 2304-8158
DOI: 10.3390/foods11091211
Popis: Ozone is widely used to control pests in grain and impacts seed germination, a crucial stage in crop establishment which involves metabolic alterations. In this study, dormancy was overcome through after-ripening (AR) in dry barley seed storage of more than 4 weeks; alternatively, a 15-min ozone treatment could break the dormancy of barley immediately after harvest, with accelerated germination efficiency remaining around 96% until 4 weeks. Headspace solid-phase microextraction (HS-SPME) and liquid absorption coupled with gas chromatography mass spectrometry (GC-MS) were utilized for metabolite profiling of 2-, 4- and 7-day germinating seeds. Metabolic changes during barley germination are reflected by time-dependent characteristics. Alcohols, fatty acids, and ketones were major contributors to time-driven changes during germination. In addition, greater fatty acids were released at the early germination stage when subjected to ozone treatment.
Databáze: Directory of Open Access Journals