Changes in plasma spectral characteristics during the in vitro contact of human venous blood with granulated sorbents
| Autor: | N. V. Burkova, O. P. Kirichuk, E. V. Romanchuk, V. A. Davankov, V. N. Postnov, S. I. Kuznetsov |
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| Jazyk: | ruština |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Alʹmanah Kliničeskoj Mediciny, Vol 46, Iss 8, Pp 772-777 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2072-0505 2587-9294 |
| DOI: | 10.18786/2072-0505-2018-46-8-772-777 |
| Popis: | Rationale: During hemosorption procedures, it is important to investigate not only the sorption and activation characteristics of hemocontact agents, but also to assess the effect of sorbents on the parameters of blood homeostasis. The intensity of hemolysis can be judged by the degree of changes in optical density of blood plasma at wavelengths corresponding to the peak absorption of hemoglobin (414, 544 and 577 nm).Aim: To assess the effect of three granular sorbents (SKT-6A, HPS, Silochrome C-120) contacting human venous blood in vitro on changes in plasma spectral characteristics.Materials and methods: The blood contact was modeled at bench conditions with the use of donated blood at rotating mode. Blood samples were drawn before the experiment and after 5, 20, 40, and 60 minutes. Spectroscopic assessment was performed in the visible light range (300–700 nm) with UNICO 2802(S) spectrophotometer.Results: The interaction of the SKT-6A sorbent with blood resulted in a 17.3% decrease in the plasma optical density at a wavelength of 540 nm, compared to baseline, as soon as at 5 minute of the experiment (p < 0.05). The decline in optical density imposed by the blood contact with HPS ranged from 2.6 to 12.1% (p < 0.05) during the observation period. The sorption activity of SKT-6A and HPS prevailed over their lytic properties. On the contrary, the percentage change in the optical density of the Silochrome C-120 sorbent during its blood contact increased from 25.6 to 38.3% (p < 0.05), indicating that this sorbent was inducing hemolysis. The sorbents tested can be arranged as follows according to their ability to induce hemolysis during their contact with blood: HPS < SKT-6A < Silochrome C-120.Conclusion: The tested SKT-6A and HPS sorbents can be used as blood-contact agents for the low volume hemoperfusion. The HPS agent seems to be the most promising for routine clinical use. The Silochrome C-120 sorbent requires some chemical modification to improve its properties of hemocompatibility. |
| Databáze: | Directory of Open Access Journals |
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