| Autor: |
Angel Vazquez-Esqueda, Rebeca Rosas-Campos, Rebeca Escutia-Gutiérrez, Ricardo de la Rosa-Bibiano, Samael Rodríguez-Sanabria, Marina Galicia-Moreno, Juan Armendáriz-Borunda, Ana Sandoval-Rodríguez |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Annals of Hepatology, Vol 29, Iss , Pp 101467- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1665-2681 |
| DOI: |
10.1016/j.aohep.2024.101467 |
| Popis: |
Introduction and Objectives: Metabolic fatty liver disease (MAFLD) is currently the most common cause of chronic liver damage worldwide. Differential methylation in genes and histones has been correlated with metabolic alterations present in the disease. Supplementation with methyl group donor molecules could work as a therapeutic strategy to reverse the progression of the disease. Materials and patients: Male C57BL/6J mice of 20-25g of initial weight were fed with a conventional diet (ND n=8); or a diet high in fat and sugar (HF n=8) for 18 weeks, or a diet high in fat and sugar for 10 weeks, plus 8 weeks of HF diet + methyl group donor supplementation (HFMS n=8). Insulin Tolerance test was performed before sacrifice. Liver, epididymal and visceral fat, and serum samples were collected. Biochemical and histological analyzes were performed. In the liver, global DNA methylation was quantified and the transcriptome was analyzed using dual-channel microarrays. Proteomic analysis was carried out by immunoblotting. Results: The supplemented animals (HFMS) showed a decrease in body weight epididymal and visceral fat (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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