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Background & aim: Nano phenol (NP) is known to be an environmental contaminant and an immunosuppressant agent, which has adverse effects on sex cell’s nucleus, inducing stress, oxidative stress and reducing fertility. The aim of the present study was to determine the protective role of Satureja khozestanica as an antioxidant on the growth process of fetuses resulting from in-vitro fertilization following Nano phenol administration. Methods: In the present experimental study conducted in 2016, 40 mature female mice, aged 6-8 weeks, weighing 20-25 grams, were randomly divided into 4 equal groups. The control group received corn oil orally and the test groups were treated with NP (225mg/kg), khozestanica essential essence (225 mg/kg), NP(225mg/kg) + khozestanica essential essence (225mg/kg) for 28 consecutive days orally by gavages. PMSG and HCG were administrated for stimulating the superovulation process. The sperms were obtained from 6 mature male mice. Following oocyte collection in-vitro fertilizing was performed using HTF+4mgBSA medium. The fertilized oocytes were incubated for 120 hours and the embryo development were studied. Two proportion test was used for statistical analyses. (p |
