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Chengjie Zhong,1,* Jiajiu Chen,2,* Yi Ling,3 Deng Liu,3 Jing Xu,2 Luofu Wang,2 Chengguo Ge,4 Qing Jiang4 1The Second Clinical Medical College, Chongqing Medical University, Chongqing, 400016, People’s Republic of China; 2Department of Urology, Daping Hospital, Army Medical University, Chongqing, 400038, People’s Republic of China; 3Department of Ultrasound, Southwest Hospital, Army Medical University, Chongqing, 400042, People’s Republic of China; 4Department of Urology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chengguo Ge; Qing Jiang, Department of Urology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, 400010, People’s Republic of China, Tel +86 13883510963 ; +86 13508346066, Email gcg2022@hospital.cqmu.edu.cn; 300899@hospital.cqmu.edu.cnBackground and Purpose: The early diagnosis and differential diagnosis of renal cell carcinoma (RCC) has always been a clinical difficulty and a research focus. Carbonic anhydrase IX (CA IX) is highly expressed on the cell membrane of RCC but is not expressed in normal renal tissues. In this study, nanobubbles (NBs) targeting CA IX with ultrasound and photoacoustic multimodal imaging capabilities were prepared to explore a new method for the diagnosis and differential diagnosis of RCC.Methods: Indocyanine green (ICG)-loaded lipid NBs (ICG-NBs) were prepared by using the filming rehydration method, and anti-CA IX polypeptides (ACPs) were attached to their surfaces to prepare CA IX-targeted NBs (ACP/ICG-NBs). The particle size, zeta potential and ICG encapsulation efficiency of these nanobubbles were measured, and their specific targeting and binding abilities to RCC cells were determined. The in vitro and in vivo ultrasound, photoacoustic and fluorescence imaging characteristics of these nanobubbles were also assessed.Results: The particle size of the ACP/ICG-NBs was 475.9 nm in diameter, and their zeta potential was − 2.65 mV. Laser confocal microscopy and flow cytometry both confirmed that ACP/ICG-NBs had specific binding activity and ideal affinity to CA IX-positive RCC cells (786-O) but not to CA IX-negative RCC cells (ACHN). The intensities of the in vitro ultrasound, photoacoustic and fluorescence imaging were positively correlated with the concentrations of ACP/ICG-NBs. In in vivo ultrasound and photoacoustic imaging experiments, ACP/ICG-NBs exhibited specific enhanced ultrasound and photoacoustic imaging effects in 786-O xenograft tumors.Conclusion: The ICG- and ACP-loaded targeted nanobubbles that we prepared had the capability of ultrasound, photoacoustic and fluorescence multimodal imaging and could specifically enhance the ultrasound and photoacoustic imaging of RCC xenograft tumors. This outcome has potential clinical application value for the diagnosis of RCC at the early stage and the differential diagnosis of benign and malignant kidney tumors.Keywords: contrast-enhanced ultrasound imaging, photoacoustic imaging, renal cell carcinoma, carbonic anhydrase IX, targeted nanobubble, fluorescence imaging |
