Validation of aqueous two-phase extraction method

Autor: Luisaldo Sandate-Flores, José Rodríguez-Rodríguez, Magdalena Rostro-Alanis, Jorge Alejandro Santiago Urbina, Karla Mayolo-Deloisa, Elda M. Melchor-Martínez, Juan Eduardo Sosa-Hernández, Roberto Parra-Saldívar, Hafiz M.N. Iqbal
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: MethodsX, Vol 8, Iss , Pp 101421- (2021)
Druh dokumentu: article
ISSN: 2215-0161
DOI: 10.1016/j.mex.2021.101421
Popis: Nowadays, consumer interest in food with natural ingredients has increased. This need has led to the research of new sources and green extraction methods. Betalains are compounds responsible for giving color to cacti fruits. The aim is to obtain low-sugar betacyanins extracts from jiotilla Escontria chiotilla using aqueous two-phase systems (ATPS) to color food with the extract. The effect of principal parameters of ATPS (Ethyl alcohol- KH2PO4/K2HPO4) as tie-line length (TL;40,50 and 70), phase volume ratios (Vr; 1 and 3) on the partitioning of betacyanins, betaxanthins, total sugars, reducing sugars, and antioxidant activity in the extract was evaluated. The yields were determined from the top and bottom phases of the aforementioned parameters. Multivariate analysis of variance (MANOVA, α = 0.05) showed that TLL and Vr were statistically significant (P
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