| Autor: |
LI Ya-fei, DU Ying-qiang, WANG Ze-mu, ZHANG Jun |
| Jazyk: |
čínština |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Jichu yixue yu linchuang, Vol 42, Iss 11, Pp 1667-1674 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1001-6325 |
| DOI: |
10.16352/j.issn.1001-6325.2022.11.1667 |
| Popis: |
Objective To explore the function and mechanism of circular RNA 0006104 (circ_0006104) in regulating proliferation, activation and extracellular matrix (ECM) synthesis of cardiac fibroblasts (CFs) induced by angiotensin Ⅱ (Ang Ⅱ). Methods The primary CFs of neonatal mice were isolated and divided into control group, circ_0006104 knockdown group, Ang Ⅱ group,Ang Ⅱ + circ_0006104 knockdown group and Ang Ⅱ + circ_0006104 knockdown+ miR-370-3p inhibitor.EDU immunofluorescence staining was used to detect the proliferation level of CFs; Western blot was used to detect activation related gene (α-SMA); RT-qPCR was used to detect the expression of collagen Ⅰ and Ⅲ (Col and Col Ⅲ). Results Circ_0006104 was screened by RNA-seq high-throughput sequencing results. In the primary CFs of neonatal mice, silencing circ_0006104 had no effect on the proliferation, activation and ECM synthesis of CFs under physiological conditions, while silencing circ_0006104 could significantly inhibit the proliferation, activation and ECM synthesis of CFs induced by Ang Ⅱ (P＜0.05). The molecules of circ_0006104 could bind to and negatively regulate miR-370-3p. The combined experimental results showed that inhibition of miR-370-3p significantly reversed the inhibitory effects of circ_0006104 silencing on the proliferation, activation and ECM synthesis of CFs induced by Ang Ⅱ (P＜0.05). Conclusions Knockdown of circ_0006104 inhibits Ang Ⅱ-induced CFs fibrosis by binding and negatively regulating miR-370-3p. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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