Chilled and post-thawed semen characteristics of buffalo semen diluted in tris extender enriched with date palm pollen grains (TPG)

Autor: Reda I. El-Sheshtawy, Walid S. El-Nattat, Samy I.A. Shalaby, Mohamed I. Shahba, Islam E. Al-Se'dawy
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Asian Pacific Journal of Reproduction, Vol 5, Iss 3, Pp 252-255 (2016)
Druh dokumentu: article
ISSN: 2305-0500
DOI: 10.1016/j.apjr.2016.04.001
Popis: Objective: The benefits of dates palm and their pollen grains in the human nourishment system have their consideration and importance in medical and nutritional point of view. The present study aimed to investigate a new perspective for the use of date palm pollen grains (DPPG) aqueous extract or infusion in preservation of chilled and frozen buffalo semen. Methods: Pooled buffalo semen were diluted by Tris-Citrate-Fructose egg yolk (TCFY) diluent (considered as control) and diverse concentrations of clarified soaked pollen grains in Tris-Citrate-Fructose (TCF) diluent in concentrations of 50, 100, 150, 200 and 250 mg DPPG/5 mL TCF. Five concentrations were kept without egg yolk (TPG) while other five concentrations contained 20% egg yolk (TPGY). Each of the two dilutions was enumerated according to the concentration of DPPG. Extended semen is then chilled (for 7 d) and cryopreserved. Motility, alive sperm and intact sperm membrane (HOST) % were recorded as characteristic sperm parameters. Results: After 7 d of chilling, significant (P
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