| Autor: |
Gabriela de Oliveira, Ismael Artur Costa-Rocha, Nani Oliveira-Carvalho, Tâmilla Mayane Alves Fidelis dos Santos, Ana Carolina Campi-Azevedo, Vanessa Peruhype-Magalhães, Vitor Hugo Simões Miranda, Roberta Oliveira Prado, Agnes Antônia Sampaio Pereira, Clarice Carvalho Alves, Joaquim Pedro Brito-de-Sousa, Laise Rodrigues Reis, Christiane Costa-Pereira, Camila Pacheco Silveira Martins da Mata, Vanessa Egídio Silveira Almeida, Liliane Martins dos Santos, Gregório Guilherme Almeida, Lis Ribeiro do Valle Antonelli, Jordana Grazziela Coelho-dos-Reis, Andréa Teixeira-Carvalho, Olindo Assis Martins-Filho |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Microorganisms, Vol 12, Iss 11, p 2272 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2076-2607 |
| DOI: |
10.3390/microorganisms12112272 |
| Popis: |
The present study aimed to evaluate the kinetics of the phenotypic profile and integrative networks of T/B-cells in severe COVID-19 patients, categorized according to disease outcome, during the circulation of the B.1.1.28 and B.1.1.33 SARS-CoV-2 strains in Brazil. Peripheral blood obtained at distinct time points (baseline/D0; D7; D14-28) was used for ex vivo flow cytometry immunophenotyping. The data demonstrated a decrease at D0 in the frequency of CD3+ T-cells and CD4+ T-cells and an increase in B-cells with mixed activation/exhaustion profiles. Higher changes in B-cell and CD4+ T-cells at D7 were associated with discharge/death outcomes, respectively. Regardless of the lower T/B-cell connectivity at D0, distinct profiles from D7/D14-28 revealed that, while discharge was associated with increasing connectivity for B-cells, CD4+ and CD8+ T-cells death was related to increased connectivity involving B-cells, but with lower connections mediated by CD4+ T-cells. The CD4+CD38+ and CD8+CD69+ subsets accurately classified COVID-19 vs. healthy controls throughout the kinetic analysis. Binary logistic regression identified CD4+CD107a+, CD4+T-bet+, CD8+CD69+, and CD8+T-bet+ at D0 and CD4+CD45RO+CD27+ at D7 as subsets associated with disease outcomes. Results showed that distinct phenotypic timeline kinetics and integrative networks of T/B-cells are associated with COVID-19 outcomes that may subsidize the establishment of applicable biomarkers for clinical/therapeutic monitoring. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
