| Autor: |
Pia Annette Johansson, Anita Adami, Johan Jakobsson |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 3, Iss 2, Pp 101285- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2022.101285 |
| Popis: |
Summary: This protocol describes the design and use of CRISPRi-mediated transcriptional silencing in human iPSCs, for loss-of-function studies in brain development research. The protocol avoids single cell selection, thereby eliminating side effects of clonal expansion and sites of viral integration. We also describe a neural progenitor differentiation protocol and discuss the challenges of target-specific lentiviral silencing, efficient silencing levels, and off-target effects.For complete details on the use and execution of this protocol, please refer to Johansson et al. (2022). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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