Expression Profile of Fas-Fas Ligand in Spiral Ganglion Cells During Apoptosis
| Autor: | Tae Woo Gu, Woo Yong Bae, Hwan Tae Park, Jae Hoon Lee, Min Young Kang, Sung Wook Jeong, Yoon Kyung Shin |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Clinical and Experimental Otorhinolaryngology, Vol 7, Iss 1, Pp 1-6 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1976-8710 2005-0720 |
| DOI: | 10.3342/ceo.2014.7.1.1 |
| Popis: | ObjectivesTo examine the expression profile of Fas-Fas ligand (FasL) during glutamate (Glu)-induced spiral ganglion cell (SGC) apoptosis.MethodsCultured SGCs were treated with 10-mM, 25-mM, and 50-mM concentrations of Glu and incubated for 24 or 48 hours. The expression intensity of FasL, Fas, caspase 3, and morphology of single SGC were evaluated using immunofluorescence staining.ResultsIn semiquantitative analysis of the Glu-treated SGC, FasL, and caspase 3 expression intensity were increased with concentration- and time-dependent manner. Fas expression intensity did not change with different concentration at 48 hours. In morphologic analysis of the Glu-treated SGC, number of apoptotic cells were increased with concentration- and time-dependent manner.ConclusionFasL was expressed in apoptotic SGCs, suggesting that the Fas-FasL signaling pathway may be involved in the Glu-induced apoptosis of dissociated SGCs. |
| Databáze: | Directory of Open Access Journals |
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