Tapered optical fiber DNA biosensor for detecting Leptospira DNA
| Autor: | Jia-Yong Lam, Mohd Hanif Yaacob, Hui-Yee Chee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Asian Pacific Journal of Tropical Medicine, Vol 16, Iss 3, Pp 119-128 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2352-4146 1995-7645 |
| DOI: | 10.4103/1995-7645.372293 |
| Popis: | Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene. Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 µm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission spectrum. Results: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/µL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification approach. Conclusions: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis. |
| Databáze: | Directory of Open Access Journals |
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