Probing human immune cell mechanics using acoustic force spectroscopy

Autor: Tom M.J. Evers, Mehrad Babaei, Alireza Mashaghi
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: STAR Protocols, Vol 5, Iss 1, Pp 102861- (2024)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2024.102861
Popis: Summary: Immune cells continuously adapt their mechanical properties for proper circulation and elicitation of immune responses. Here, we provide a step-by-step protocol for probing the single-cell mechanical properties of primary human monocytes using acoustic force spectroscopy (AFS). We describe steps for the calibration of the AFS chips, the isolation of monocytes from buffy coats, and the probing of monocyte mechanics using AFS. We then detail the data analysis strategy. The protocol is useful for characterizing a wide range of immune cells under various conditions in physiology and pathology.For complete details on the use and execution of this protocol, please refer to Evers et al. 1 and Evers et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Databáze: Directory of Open Access Journals