Modulation of stem cell response using biodegradable polyester films with different stiffness
| Autor: | Sofia Ribeiro, Eugenia Pugliese, Stefanie H. Korntner, Emanuel M. Fernandes, Manuela E. Gomes, Rui L. Reis, Yves Bayon, Dimitrios I. Zeugolis |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Biomedical Engineering Advances, Vol 2, Iss , Pp 100007- (2021) |
| Druh dokumentu: | article |
| ISSN: | 2667-0992 25062182 |
| DOI: | 10.1016/j.bea.2021.100007 |
| Popis: | Matrix stiffness is a crucial regulator of cell fate in both in vitro and in vivo setting. Although studies with non-degradable polymers have contributed to our understanding of the influence of rigidity on cell response, very little work has been conducted with biodegradable polymers that constitute the building blocks of implantable devices. Herein, we investigated human bone marrow stem cell response as a function of rigidity (7 kPa, 10 kPa, 12 kPa, 22 kPa, 15 MPa elastic modulus values) that induced by five different aliphatic polyesters (tissue culture plastic with ~ 3 GPa rigidity was used as control). Cell morphology analysis revealed that stiff substrates stimulated a large cell area with defined stress fibres, whilst soft substrates prompted a small cell area without evident stress fibres. Immunocytochemistry analysis made apparent that YAP was accumulated at the nuclei when the cells were seeded on stiff substrates and at the cytoskeleton on soft substrates. Substrate stiffness did not affect (p > 0.05) the expression of positive (> 97% CD73, CD90, CD105, CD44), but increased (p |
| Databáze: | Directory of Open Access Journals |
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