Use of ultrasonication to increase germination rates of Arabidopsis seeds

Autor: Ignacio López-Ribera, Carlos M. Vicient
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Plant Methods, Vol 13, Iss 1, Pp 1-6 (2017)
Druh dokumentu: article
ISSN: 1746-4811
DOI: 10.1186/s13007-017-0182-6
Popis: Abstract Background Arabidopsis thaliana is widely used as model organism in plant biology. Although not of agronomic significance, it offers important advantages for basic research in genetics and molecular biology including the availability of a large number of mutants and genetically modified lines. However, Arabidopsis seed longevity is limited and seeds stored for more than 10 years usually show a very low capacity for germination. Results The influence of ultrasonic stimulation was investigated on the germination of A. thaliana L. seeds. All experiments have been performed using a frequency of 45 kHz at constant temperature (24 °C). No germination rate differences were observed when using freshly collected seeds. However, using artificially deteriorated seeds, our results show that short ultrasonic stimulation (
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