Exploration of Mechanistic Insights of Acemetacin in Melanogenesis Through Zebrafish Model, Enzyme Kinetics, Molecular Docking and Simulation Approaches
| Autor: | Hussain Raza, Mehar Ali Kazi, Mubashir Hassan, Qamar Abbas, Sung-Yum Seo |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Pakistan Journal of Analytical & Environmental Chemistry, Vol 21, Iss 1, Pp 115-124 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1996-918X 2221-5255 |
| DOI: | 10.21743/pjaec/2020.06.14 |
| Popis: | The present study describes the anti‐melanogenesis effect of Acemetacin (ACE). Essential protein (melanin) that is vital for the skin for defense from UV rays. In the present research, emerging drug ACE was examined for its melanin inhibition using three different (in vitro, in vivo and computational) methods. ACE showed remarkable potency (IC50 = 0.353 ± 0.003 µM) against tyrosinase in the comparison of standard, kojic acid (IC50 = 16.841 ± 1.161 µM) and ACE exhibited competitive inhibition. In the in vivo study zebrafish embryos were exposed with 5, 10, 15 and 20 µM of ACE and same doses for positive control (Kojic Acid). At 72 h treatment, ACE expressively (P |
| Databáze: | Directory of Open Access Journals |
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