Association of Ovar‐DRB1 alleles with innate immune responses in sheep

Autor: Atefeh Esmailnejad, Vahid Ganjiani, Elhamsadat Hosseini‐Nasab, Saeed Nazifi
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Veterinary Medicine and Science, Vol 8, Iss 2, Pp 752-757 (2022)
Druh dokumentu: article
ISSN: 2053-1095
DOI: 10.1002/vms3.683
Popis: Abstract Background Major histocompatibility complex (MHC) is the best characterised genetic region associated with adaptive immune responses, including humoral and cell‐mediated immunities. Objectives In this study, the association of MHC class II alleles with inflammatory cytokines and acute‐phase proteins was evaluated in sheep population. Methods Allelic diversity of second exon of ovine DRB1 locus (Ovar‐DRB1.2) was determined in 100 indigenous Iranian Lori‐Bakhtiari fat‐tailed sheep using restriction fragment length polymorphism and direct sequencing methods. The association of DRB1.2 alleles with inflammatory cytokines (interleukin‐1β, IL‐1β; IL‐6 and tumour necrosis factor‐α) and acute‐phase proteins (serum amyloid A, alpha‐1‐acid glycoprotein and haptoglobin) was examined using generalised linear model and multivariate regression analysis. Results Seven distinct RsaI restriction patterns and fourteen alleles were identified in this population. Allele DRB1*2101 showed a negative influence on the IL‐6 response and was associated with lower serum level of IL‐6. DRB1.2 heterozygous individuals also showed higher haptoglobin concentration than homozygotes. Conclusions These results provide additional support for the association between Ovar‐DRB1 alleles and regulation of immune responses in sheep population. Description of MHC polymorphism and its role in the controlling of immune responses will increase our understanding of host–pathogen interactions, and ultimately facilitate the selection of disease‐resistant flocks in genetic breeding programs.
Databáze: Directory of Open Access Journals
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje