| Autor: |
Qianyi Deng, Qin Liu, Huini Zhang, Wenguo Fan, Jingzhou Li, Jun Kang, Hongwen He, Fang Huang |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Journal of Dental Sciences, Vol 14, Iss 4, Pp 370-377 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1991-7902 |
| DOI: |
10.1016/j.jds.2019.05.003 |
| Popis: |
Background/purpose: Melatonin, at physiological concentrations, was previously found to inhibit proliferation and promote odontogenic differentiation in human dental pulp cells (hDPCs), but its effect on apoptosis is unclear. Our study aimed to investigate the effect of melatonin on the H2O2-mediated viability reduction and apoptosis in hDPCs. Materials and methods: hDPCs were treated with H2O2 (0, 250, 500, 1000 μmol/L), melatonin (0, 10−12, 10−10, 10−8 mol/L), and melatonin with H2O2 for 24 h. CCK-8 assays were performed to evaluate cell viability. Apoptosis was measured by DAPI and Annexin V/propidium iodide staining. Intracellular reactive oxygen species (ROS) were measured by CellROX® staining and mitochondrial membrane potential (ΔΨm) was examined by JC-1 staining. Results: H2O2 obviously decreased the viability of hDPCs in a concentration-dependent manner and melatonin alone also reduced viability by 16–20%. Melatonin was also found to enhance H2O2-induced toxicity in a concentration-dependent manner, and the highest physiological concentration of melatonin (10−8 mol/L) had the most obvious effect (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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