| Autor: |
Fernando Henrique Antunes Murata, Mariana Previato, Fábio Batista Frederico, Amanda Pires Barbosa, Fabiana Nakashima, Geraldo Magela de Faria, Aparecida Perpétuo Silveira Carvalho, Cristina da Silva Meira Strejevitch, Vera Lucia Pereira-Chioccola, Lilian Castiglioni, Luiz Carlos de Mattos, Rubens Camargo Siqueira, Cinara Cássia Brandão de Mattos |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Frontiers in Cellular and Infection Microbiology, Vol 9 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2235-2988 |
| DOI: |
10.3389/fcimb.2019.00472 |
| Popis: |
Ocular toxoplasmosis is one of the most common complications caused by the infection with the parasite Toxoplasma gondii. The risk of developing eye lesions and impaired vision is considered higher in Brazil than other countries. The clinical diagnosis is difficult and the use of sensitive and specific laboratorial methods can aid to the correct diagnosis of this infection. We compared serological methods ELISA and ELFA, and molecular cPCR, Nested PCR and qPCR for the diagnosis of T. gondii infection in groups of patients clinically evaluated with ocular diseases non-toxoplasma related (G1 = 185) and with lesions caused by toxoplasmosis (G2 = 164) in an Ophthalmology clinic in Brazil. Results were compared by the Kappa index, and sensitivity (S), specificity (E), positive predictive value (PPV), and negative (NPV) were calculated. Serologic methods were in agreement with ELISA more sensitive and ELFA more specific to characterize the acute and chronic infections while molecular methods were discrepant where qPCR presented higher sensitivity, however, lower specificity when compared to cPCR and Nested PCR. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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