High‐Speed Centrifugation Efficiently Removes Immunogenic Elements in Osteochondral Allografts

Autor: Yongsheng Ma, Qitai Lin, Wenming Yang, Yang Liu, Yugang Xing, Zhiyuan Ren, Xueding Wang, Raorao Zhou, Gaige Wu, Pengcui Li, Wangping Duan, Xiaoling Zhang, Xiaochun Wei
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Orthopaedic Surgery, Vol 16, Iss 3, Pp 675-686 (2024)
Druh dokumentu: article
ISSN: 1757-7861
1757-7853
DOI: 10.1111/os.13991
Popis: Objectives The current clinical pulse lavage technique for flushing fresh osteochondral allografts (OCAs) to remove immunogenic elements from the subchondral bone is ineffective. This study aimed to identify the optimal method for removing immunogenic elements from OCAs. Methods We examined five methods for the physical removal of immunogenic elements from OCAs from the femoral condyle of porcine knees. We distributed the OCAs randomly into the following seven groups: (1) control, (2) saline, (3) ultrasound, (4) vortex vibration (VV), (5) low‐pulse lavage (LPL), (6) high‐pulse lavage (HPL), and (7) high‐speed centrifugation (HSC). OCAs were evaluated using weight measurement, micro‐computed tomography (micro‐CT), macroscopic and histological evaluation, DNA quantification, and chondrocyte activity testing. Additionally, the subchondral bone was zoned to assess the bone marrow and nucleated cell contents. One‐way ANOVA and paired two‐tailed Student's t‐test are used for statistical analysis. Results Histological evaluation and DNA quantification showed no significant reduction in marrow elements compared to the control group after the OCAs were treated with saline, ultrasound, or VV treatments; however, there was a significant reduction in marrow elements after LPL, HPL, and HSC treatments. Furthermore, HSC more effectively reduced the marrow elements of OCAs in the middle and deep zones compared with LPL (p
Databáze: Directory of Open Access Journals
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje