Organotypic Spinal Cord Slice Cultures and a Method to Detect Cell Proliferation in These Slices

Autor: Jillian Daniel, Jim Deuchars, Susan Deuchars
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Bio-Protocol, Vol 6, Iss 19 (2016)
Druh dokumentu: article
ISSN: 2331-8325
DOI: 10.21769/BioProtoc.1951
Popis: In these culture models, the normal cytoarchitecture and local neuronal circuits of the spinal cord are preserved, offering a compromise between dissociated cell cultures and complete animal studies. The addition of 5-ethynyl-2’-deoxyuridine (EdU) to the culture medium allows for the detection of proliferating cells.
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