| Autor: |
Y. Oshima-Franco, C. M. V. Alves, N. Andréo Filho, M. Gerenutti, A. C. O. Cintra, G. B. Leite, L. Rodrigues-Simioni, M. G. Silva |
| Jazyk: |
angličtina |
| Rok vydání: |
2005 |
| Předmět: |
|
| Zdroj: |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 4, Pp 465-478 (2005) |
| Druh dokumentu: |
article |
| ISSN: |
1678-9199 |
| DOI: |
10.1590/S1678-91992005000400007 |
| Popis: |
Numerous plants are used as snakebite antidotes in Brazilian folk medicine, including Casearia sylvestris Swartz, popularly known as guaçatonga. In this study, we examined the action of a hydroalcoholic extract from C. sylvestris on the neuromuscular blockade caused by bothropstoxin-I (BthTX-I), a myotoxin from Bothrops jararacussu venom, in mouse isolated phrenic nerve-diaphragm (PND) preparations. Aqueous (8 and 12 mg/ml, n=4 and 5, respectively) and hydroalcoholic (12 mg/ml, n=12) extracts of the leaves of C. sylvestris caused facilitation in PND preparations followed by partial neuromuscular blockade. BthTX-I (20 µg/ml, n=4) caused 50% paralysis after 65±15 min (mean ± S.E.M). Preincubation (30 min at 37° C) of BthTX-I (20 µg/ml, n=4) with a concentration of the hydroalcoholic extract (4 mg/ml) that had no neuromuscular activity, such as the control (n=5), prevented the neuromuscular blockade caused by the toxin. This protection may be mediated by compounds such as flavonoids and phenols identified by thin-layer chromatography and colorimetric assays. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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