| Autor: |
Max Koppers, Roberta Cagnetta, Toshiaki Shigeoka, Lucia CS Wunderlich, Pedro Vallejo-Ramirez, Julie Qiaojin Lin, Sixian Zhao, Maximilian AH Jakobs, Asha Dwivedy, Michael S Minett, Anaïs Bellon, Clemens F Kaminski, William A Harris, John G Flanagan, Christine E Holt |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
eLife, Vol 8 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2050-084X |
| DOI: |
10.7554/eLife.48718 |
| Popis: |
Extrinsic cues trigger the local translation of specific mRNAs in growing axons via cell surface receptors. The coupling of ribosomes to receptors has been proposed as a mechanism linking signals to local translation but it is not known how broadly this mechanism operates, nor whether it can selectively regulate mRNA translation. We report that receptor-ribosome coupling is employed by multiple guidance cue receptors and this interaction is mRNA-dependent. We find that different receptors associate with distinct sets of mRNAs and RNA-binding proteins. Cue stimulation of growing Xenopus retinal ganglion cell axons induces rapid dissociation of ribosomes from receptors and the selective translation of receptor-specific mRNAs. Further, we show that receptor-ribosome dissociation and cue-induced selective translation are inhibited by co-exposure to translation-repressive cues, suggesting a novel mode of signal integration. Our findings reveal receptor-specific interactomes and suggest a generalizable model for cue-selective control of the local proteome. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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