| Autor: |
Monir M. M. El Husseini |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Egyptian Journal of Biological Pest Control, Vol 29, Iss 1, Pp 1-4 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2536-9342 |
| DOI: |
10.1186/s41938-019-0159-z |
| Popis: |
Abstract Larvae of the beet web moth, Spoladea recurvalis F. (Lepidoptera: Crambidae), were reared on a semi-synthetic diet under laboratory temperature of 25 °C. The microbial bioinsecticide Dipel 2X (Bacillus thuringiensis kurstaki) was bio-assayed versus the 2nd instar larvae (L2) of S. recurvalis by mixing in the semi-synthetic diet at different concentrations (5, 2.5, 1.25; 0.6 and 0.3% w/w). The concentration 1.25% of Dipel 2X mixed in the semi-synthetic diet was fed to larvae (L2) to prolong the pre-death period for 5 days in order to follow its effect on the structure of mid-gut. Treated larvae as well as those of the control were fixed in Bouin’s fixative at 1, 2, 3, 4, and 5 days post treatment and processed for paraffin serial sectioning at 6–8 μm with double staining by eosin and hematoxylin. Cross sections in the mid-gut were examined with a light microscope in both treated larvae and those of the control. The gradual alterations in mid-gut structure till death of the treated larvae were described. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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