Infection with multiple types of human papillomavirus in sexually active young women
| Autor: | Ana Paula Machado de Almeida, Ana Paula Machado, Flávia Gatto de Almeida, Leandro Sobrinho Ávilla, Thiago Theodoro Martins Prata, Larissa Zatorre Almeida, Camila Mareti Bonin, Cacilda Tezelli Junqueira Padovani, Alda Maria Teixeira Ferreira, Carlos Eurico dos Santos Fernandes, Inês Aparecida Tozetti |
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| Jazyk: | portugalština |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Medicina, Vol 48, Iss 6 (2015) |
| Druh dokumentu: | article |
| ISSN: | 2176-7262 0076-6046 |
| DOI: | 10.11606/issn.2176-7262.v48i6p573-579 |
| Popis: | Study design: cross. Study objective: estimate the frequency and distribution types of human papillomavirus (HPV) in sexually active young women. Methods: samples of vaginal and endocervical canal of 158 women, aged between 18 and 35 years were collected through self-collection technique. The HPV DNA was extracted and amplified by PCR end point using the consensus primers PGMY09/11 and genotyping was performed by PCR specific type (TS-PCR) and by analysis of the fragments obtained from the use of restriction enzymes (RFLP). Results: HPV DNA was detected in 23% of participants, 92% were high-risk HPV genotypes (HR), being the prevalent HPV45, followed by HPV16 and 31 infections with multiple HPV types were detected in 35% of samples, indicating infection by more than two types in 22.2% of the genotyped. Higher frequency of HPV DNA was detected in women ≤ 25 old. Conclusion: this demonstrated a high frequency of HPV infection in sexually active young women, as well as a high prevalence of infection with multiple types of high oncogenic risk |
| Databáze: | Directory of Open Access Journals |
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