Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling

Autor: Anna M. Kotowska, Gustavo F. Trindade, Paula M. Mendes, Philip M. Williams, Jonathan W. Aylott, Alexander G. Shard, Morgan R. Alexander, David J. Scurr
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Nature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-020-19445-x
Popis: Label-free protein characterization at surfaces requires digestion or matrix application prior to mass spectrometry. Here, the authors report the assignment of undigested proteins at surfaces by de novo sequencing and apply the methodology to a protein monolayer biochip and for in situ depth profiling of proteins through human skin.
Databáze: Directory of Open Access Journals