A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation

Autor: Fuad Gandhi Torizal, Qiao You Lau, Masato Ibuki, Yoshikazu Kawai, Masato Horikawa, Masataka Minami, Tatsuo Michiue, Ikki Horiguchi, Masaki Nishikawa, Yasuyuki Sakai
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Communications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
Druh dokumentu: article
ISSN: 2399-3642
DOI: 10.1038/s42003-021-02848-x
Popis: Although human pluripotent stem cell culture is routine, daily media changes, high cost of growth factors required in pluripotent cell media, and reproducibility of differentiation are an obstacle for the mass production of hPSCs. Here, Torizal et al. describe a cost-effective dialysis-culture system for human pluripotent stem cells (hPSCs) at a high density.
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