High‐Throughput and Dosage‐Controlled Intracellular Delivery of Large Cargos by an Acoustic‐Electric Micro‐Vortices Platform

Autor:	Mohammad Aghaamoo, Yu‐Hsi Chen, Xuan Li, Neha Garg, Ruoyu Jiang, Jeremy Tian‐Hao Yun, Abraham Phillip Lee
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	CRISPR‐Cas9 intracellular delivery large cargo precise‐dose delivery Science
Zdroj:	Advanced Science, Vol 9, Iss 1, Pp n/a-n/a (2022)
Druh dokumentu:	article
ISSN:	2198-3844
DOI:	10.1002/advs.202102021
Popis:	Abstract A high‐throughput non‐viral intracellular delivery platform is introduced for the transfection of large cargos with dosage‐control. This platform, termed Acoustic‐Electric Shear Orbiting Poration (AESOP), optimizes the delivery of intended cargo sizes with poration of the cell membranes via mechanical shear followed by the modulated expansion of these nanopores via electric field. Furthermore, AESOP utilizes acoustic microstreaming vortices wherein up to millions of cells are trapped and mixed uniformly with exogenous cargos, enabling the delivery of cargos into cells with targeted dosages. Intracellular delivery of a wide range of molecule sizes (90%), cell viability (>80%), and uniform dosages (
Databáze:	Directory of Open Access Journals
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