Diagnosis of genitourinary tuberculosis by loop-mediated isothermal amplification based on SYBR Green I dye reaction
Autor: | Ekta Kamra, Netrapal Singh, Anish Khan, Jaideep Singh, Meenakshi Chauhan, Hemant Kamal, Promod K Mehta |
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Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: | |
Zdroj: | BioTechniques, Vol 73, Iss 1, Pp 47-57 (2022) |
Druh dokumentu: | article |
ISSN: | 1940-9818 0736-6205 |
DOI: | 10.2144/btn-2022-0027 |
Popis: | A multitargeted loop-mediated isothermal amplification (MT-LAMP) assay targeting mpt64 (Rv1980c) and IS6110 was designed to diagnose genitourinary tuberculosis (GUTB) cases. While assessing gel-based, hydroxynaphthol blue (HNB) and SYBR Green I MT-LAMP assays on GUTB specimens (n = 28) in a pilot study, both gel-based/SYBR Green I assays exhibited better sensitivity than HNB LAMP. Since SYBR Green MT-LAMP is easier to perform compared with a gel-based assay, a higher number of GUTB specimens (n = 55) were evaluated by SYBR Green MT-LAMP, wherein 85.5% sensitivity and 94.4% specificity (n = 36) were obtained. Moreover, the sensitivity attained by MT-LAMP was significantly higher (p |
Databáze: | Directory of Open Access Journals |
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