Degradation and inactivation of adenovirus in water by photo-electro-oxidation
| Autor: | G. S. Monteiro, R. Staggemeier, C. R. Klauck, A. M. Bernardes, M. A. S. Rodrigues, F. R. Spilki |
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| Jazyk: | angličtina |
| Předmět: | |
| Zdroj: | Brazilian Journal of Biology, Vol 75, Iss 4 suppl 2, Pp 37-42 |
| Druh dokumentu: | article |
| ISSN: | 1678-4375 1519-6984 |
| DOI: | 10.1590/1519-6984.00813suppl |
| Popis: | The present study analyzed the efficiency of the photo-electro-oxidation process as a method for degradation and inactivation of adenovirus in water. The experimental design employed a solution prepared from sterile water containing 5.107 genomic copies/L (gc/L) of a standard strain of human adenovirus type 5 (HAdV-5) divided into two equal parts, one to serve as control and one treated by photo-electro-oxidation (PEO) for 3 hours and with a 5A current. Samples collected throughout the exposure process were analyzed by real-time polymerase chain reaction (qPCR) for viral genome identification and quantitation. Prior to gene extraction, a parallel DNAse treatment step was carried out to assess the integrity of viral particles. Integrated cell culture (ICC) analyses assessed the viability of infection in a cell culture. The tested process proved effective for viral degradation, with a 7 log10 reduction in viral load after 60 minutes of treatment. The DNAse-treated samples exhibited complete reduction of viral load after a 75 minute exposure to the process, and ICC analyses showed completely non-viable viral particles at 30 minutes of treatment. |
| Databáze: | Directory of Open Access Journals |
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