Simple, inexpensive and RNase-free purification of plasmid DNA by fractional precipitation with isopropanol

Autor:	Viiu Paalme, Mart Speek
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	alkaline lysis fractional precipitation isopropanol lipopolysaccharides plasmid DNA RNase Biology (General) QH301-705.5
Zdroj:	BioTechniques, Vol 71, Iss 3, Pp 490-494 (2021)
Druh dokumentu:	article
ISSN:	1940-9818 0736-6205
DOI:	10.2144/btn-2021-0018
Popis:	We present a modified alkaline lysis method for purification of plasmid DNA (pDNA) from bacterial extract using fractional precipitation with isopropanol (FPI). This method includes two successive precipitations with 0.33 and 0.36 volumes of isopropanol and separates pDNA from total RNA and most of the lipopolysaccharides. Using different quality control tests, we demonstrate that plasmids purified with FPI show superior quality compared to plasmids prepared with commercial kits based on spin-column chromatography.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/9a3f914ae8104e6281caeb59a24eb868 Zobrazit plný text záznamu View record in DOAJ