Estimation of Early Postmortem Interval of Asphyxial Death Rats at Different Ambient Temperatures by GC-MS-Based Metabolomics

Autor: FANG Shi-yong, DAI Xin-hua, XIAO Li, et al.
Jazyk: čínština
Rok vydání: 2020
Předmět:
Zdroj: Fayixue Zazhi, Vol 36, Iss 6, Pp 741-748 (2020)
Druh dokumentu: article
ISSN: 1004-5619
DOI: 10.12116/j.issn.1004-5619.2020.06.001
Popis: Objective To establish the orthogonal partial least square (OPLS) model for the estimation of early postmortem interval (PMI) of asphyxial death rats in four ambient temperatures based on gas chromatography-mass spectrometry (GC-MS) metabolomics. Methods The 96 rats were divided into four temperature groups (5 ℃, 15 ℃, 25 ℃ and 35 ℃). Each temperature group was further divided into 3 h, 6 h, 12 h and 24 h after death, and 6 other rats were taken as the control group. The cardiac blood was collected at the set time points for the four temperature groups and 0 h after death for the control group for the metabolomics analysis by GC-MS. By OPLS analysis, the variable importance in projection (VIP)>1 and the result of Kruskal-Wallis test P
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