CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering

Autor:	Daniel Paolo Anderson, Henry James Benns, Edward William Tate, Matthew Andrew Child
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	CRISPR genome engineering protein‐centric target prioritization Biology (General) QH301-705.5 Medicine (General) R5-920
Zdroj:	Molecular Systems Biology, Vol 16, Iss 6, Pp 1-7 (2020)
Druh dokumentu:	article
ISSN:	1744-4292
DOI:	10.15252/msb.20209475
Popis:	Abstract Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target specific residues, or amino acid types within proteins. gRNA outputs can be customized to support maximal efficacy of homology‐directed repair for engineering purposes, removing time‐consuming post hoc curation, simplifying gRNA outputs and reducing CPU times.
Databáze:	Directory of Open Access Journals
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