| Autor: |
Bowen Zhang, Dinuo Zheng, Shi Yiming, Kazuhiro Oyama, Masahiro Ito, Masaomi Ikari, Takanori Kigawa, Tsutomu Mikawa, Takeo Miyake |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Small Science, Vol 1, Iss 12, Pp n/a-n/a (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2688-4046 |
| DOI: |
10.1002/smsc.202100069 |
| Popis: |
Hollow nanostructures combined with electroporation are potentially valuable in interdisciplinary fields due to their ability to transport versatile cargos into adhesive cells. However, they require voltages over 1.5 V to electroporate the physical barrier of the cell membrane inducing cell death and differentiation processes. Intracellular delivery is exhibited using a metal–organic hybrid nanotube (NT) stamp that physically inserts into the cells and subsequently injects versatile molecules at an extremely low voltage of ±50 mV (less than membrane potential). The hybrid NTs consist of Au NTs polymerizing electrochemically 3,4‐ethylenedioxythiophene monomer and supportive polycarbonate membrane. The hybrid stamp improves the cell viability by 94% for a 30 min physical insertion while decreasing the cell viability to 1% using the original Au NTs. Furthermore, the hybrid stamp acts as an electrochemical gate that can open the pore at ±50 mV to transport small molecules of calcein dye with high efficiency (99%) and viability (96.8%). The hybrid nanogate can also transport large molecules of green fluorescent protein (GFP) with 84% efficiency and 98.5% viability, and GFP plasmid at a transfection rate of ≈10%. Thus, the present hybrid stamping can potentially deliver versatile molecules into adhesive cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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