| Autor: |
Qian Yang, Xixi Cai, Muchen Huang, Shaoyun Wang |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Journal of Functional Foods, Vol 68, Iss , Pp 103887- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1756-4646 |
| DOI: |
10.1016/j.jff.2020.103887 |
| Popis: |
Pseudostellaria heterophylla protein hydrolysate has been reported to be a potential nutraceutical supplement to strengthen the immune system. A specific peptide from P. heterophylla protein hydrolysate with proliferation promotion effect on spleen lymphocytes was purified by RP-HPLC. The amino acid sequence of the peptide was determined to be RGPPP, with molecular weight of 522.29 Da. 100 μg/mL of RP-5 achieved the highest stimulation index of 1.27 on spleen lymphocytes and activated RAW264.7 cells resulting in up-regulating tumor necrosis factor-α (TNF-α) secretion to 827.24 pg/mL and elevating pinocytosis rate to 123.81%. Results of western-blot and siRNA experiments indicated that RP-5 activated nuclear factor-κB (NF-κB) signaling pathway through combining to TLR2 expressed on RAW264.7 cells surface. Furthermore, TNF-α secretion increased by RP-5 could be decreased to 401.68 pg/mL by pyrrolidine dithiocarbamate or BAY11-7082. These findings further the research progress of peptide with immunomodulatory activity and its action mechanism. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect