| Autor: |
Mi-Hyeon Jang, Min-Chul Shin, Sabina Lim, Seung-Moo Han, Hi-Joon Park, Insop Shin, Ji-Suk Lee, Kyoung-Ah Kim, Ee-Hwa Kim, Chang-Ju Kim |
| Jazyk: |
angličtina |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Journal of Pharmacological Sciences, Vol 91, Iss 2, Pp 95-104 (2003) |
| Druh dokumentu: |
article |
| ISSN: |
1347-8613 |
| DOI: |
10.1254/S1347-8613(19)32749-5 |
| Popis: |
To investigate whether bee venom (BV) induces apoptosis, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase- mediated dUTP nick end-labeling assay, 4,6-diamidino-2-phenylindole staining, flow cytometric analysis, and DNA fragmentation assay were performed on NCI-H1299 lung cancer cells treated with BV. Through morphological and biochemical analyses, it was demonstrated that NCI-H1299 cells treated with BV exhibit several features of apoptosis. In addition, reverse transcription-polymerase chain reaction and prostaglandin E2 (PGE2) immunoassay were performed to verify whether BV possesses an inhibitory effect on the expression of cyclooxygenase (COX) and PGE2 synthesis. Expression of COX-2 mRNA and synthesis of PGE2 were inhibited by BV. These results suggest the possibility that BV may exert an anti-tumor effect on human lung cancer. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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