Effect of fruit juices in skim milk extender in maintaining Sapudi ram spermatozoa quality at chilled temperature

Autor: Ayun Tria Marga Retta, Suherni Susilowati, Sri Pantja Madyawati, Tatik Hernawati, Wurlina Wurlina, Retno Sri Wahjuni
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Ovozoa: Journal of Animal Reproduction, Vol 11, Iss 2, Pp 49-53 (2022)
Druh dokumentu: article
ISSN: 2302-6464
2722-967X
DOI: 10.20473/ovz.v11i2.2022.49-53
Popis: This study aimed to determine the effect of the addition of cherry tomato, california papaya, and honey melon juice into skim milk extender in maintaining the quality of Sapudi ram semen at chilled temperature (5oC) storage. Five replication of ejaculates were divided equally into four groups. In control group (T0) semen was diluted in skim milk extender, while in T1, T2, and T3 groups semen were diluted in skim milk extender contained 20% of cherry tomato, california papaya, and honey melon juice, respectively. The extended semen was stored at a chilled temperature, and semen quality (based on sperm motility, viability, and plasma membrane integrity) was observed daily for five days. The result showed that semen quality declined day by day during the five days of storage. Based on the minimum standard of post-thawed semen motility (40%), the spermatozoa of the control group only lasted by the second day. Meanwhile, in the groups with the addition of fruit juice motility could last up to the third day, with the highest motility (p
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