Interleukin-1 beta and interleukin-8 in healthy and inflamed dental pulps
| Autor: | Alessandra Cecília Oliveira Silva, Miriam Rubio Faria, Alexandra Fontes, Marcia Sampaio Campos, Bruno Neves Cavalcanti |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | Journal of Applied Oral Science, Vol 17, Iss 5, Pp 527-532 (2009) |
| Druh dokumentu: | article |
| ISSN: | 1678-7757 1678-7765 |
| DOI: | 10.1590/S1678-77572009000500031 |
| Popis: | After aggression to the dental pulp, some cells produce cytokines in order to start and control the inflammatory process. Among these cytokines, interleukin-1 beta (IL-1β) and interleukin-8 (IL-8) emerge as important ones. OBJECTIVE: The purpose of this study was to analyze the location, distribution and concentration of these cytokines in healthy and inflamed dental pulps. MATERIAL AND METHODS: Twenty pulps, obtained from healthy third molars (n=10) and from pulpectomies (n=10) were used for the study, with half of each group used for immunohistochemistry and half for protein extraction and ELISA assays. Fibroblasts obtained from healthy dental pulps, stimulated or not by Escherichia coli lipopolysaccharide (LPS), in order to simulate aggression on the cell cultures, were also used and analyzed by ELISA for IL-1β and IL-8 as complementary information. Data obtained from immunohistochemistry were qualitatively analyzed. Data obtained from ELISA assays (tissue and cells) were statistically treated by the t-test (p |
| Databáze: | Directory of Open Access Journals |
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