In vitro co-culture models for the assessment of orthopedic antibacterial biomaterials

Autor: Benedictus I. M. Eijkel, Iulian Apachitei, Lidy E. Fratila-Apachitei, Amir A. Zadpoor
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
Druh dokumentu: article
ISSN: 2296-4185
DOI: 10.3389/fbioe.2024.1332771
Popis: The antibacterial biofunctionality of bone implants is essential for the prevention and treatment of implant-associated infections (IAI). In vitro co-culture models are utilized to assess this and study bacteria-host cell interactions at the implant interface, aiding our understanding of biomaterial and the immune response against IAI without impeding the peri-implant bone tissue regeneration. This paper reviews existing co-culture models together with their characteristics, results, and clinical relevance. A total of 36 studies were found involving in vitro co-culture models between bacteria and osteogenic or immune cells at the interface with orthopedic antibacterial biomaterials. Most studies (∼67%) involved co-culture models of osteogenic cells and bacteria (osteo-bac), while 33% were co-culture models of immune cells and bacterial cells (im-bac). All models involve direct co-culture of two different cell types. The cell seeding sequence (simultaneous, bacteria-first, and cell-first) was used to mimic clinically relevant conditions and showed the greatest effect on the outcome for both types of co-culture models. The im-bac models are considered more relevant for early peri-implant infections, whereas the osteo-bac models suit late infections. The limitations of the current models and future directions to develop more relevant co-culture models to address specific research questions are also discussed.
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